Gas chromatography mass spectrum analysis of methanolic extract of the filtrate of T. harzianum revealed the presence of nineteen bioactive compounds were identified in the methanolic extract of Trichoderma horzianum. GC-MS analysis of Trichoderma horzianum revealed the existence of the ?-D-Glucopyranose , 1-thio-,1-[N-hydroxy-5-(methylthio)pentanim , 6-Acetyl-?-d-mannose , 17-Octadecynoic acid , Paromomycin ,Imidazole , 2-amino-5-[(2-carboxy)vinyl]- , D-Glucose , 6-O-?-D-galactopyranosyl- ,?-D-Glucopyranoside,O-?-D-glucopyranosyl-(1.fwdarw.3)-?-D-, Cyclohexanecarbo-xylic acid , 2-hydroxy-1,6-dimethyl-,methyl ester , 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- , 2H-Pyran,tetrahydro-2-(12pentadecynyloxy)- , 5-Hydroxymethylfurfural , 2-Oxabicyclo[3.3.0]oct-7-en-3-one , 7-(1-hydroxypentyl)- , Dodecanoic acid , 3-hydroxy- , 1-Gala-l-ido-octonic lactone , 1,2,4-trioxolane-2-octanoic acid , 5-octyl-,methyl ester , Acetamide , N-methyl-N-[4-[2-acetoxymethyl-1-pyrrolidyl]-2-butyn , 2,5,5,8a-Tetramethyl-6,7,8,8a-tetrahydro-5H-chromen-8-ol , 5H-Cyclopropa[3,4]benz [1,2-e]azulen-5-one,9-(acetyloxy)-3- and 9,10-Secocholesta-5,7,10(19)-triene3,24, 25-triol,(3?,5Z,7E). Lycium afrum was very highly antifungal activity (6.97±0.25) mm. The results of anti-bacterial activity produced by T. horzianum showed that the volatile compounds were highly effective to suppress the growth of E. coli