The aims of our research were analysis of the secondary rnetabolite products and in vitro antibacterial and
anti-fungal activities. lnfectious diseases which are one of the main causes of morbidity and mortality
worldwide represent a critical problem to health. Pharmacological industries have produced number of newantibiotics
in the last three decades, but microbial resistance to these antibiotics has increased because of
genetic ability of the bacteria to acquire and kansmit the resistance against therapeutic agents. Mentha
pulegium is a species of flowering plant in the family Lamiaceae. The FTIR analysis of Mentha pulegium
leaves proved the presence of alkenes, alkyl halides, aromatic, and amide which shows major peaks at
958.62,985.62,1012.63,1026.13,1093.64, 1242.16,1255.66,1593.20,1606.70 and2362"80.Inthecurrent
study, the anti-microbial activity af Mentlta pulegium methanolic extract was evaluated by determining the
zone of inhibition against bacteria, fungi and yeast. Maximum zone formation was against Staphylococcus
aureus (5.89*0.20). MenthapulegtumwasveryhighlyactiveagainstAspergillusterreus{fi7*A.22).