Al-Qadisiyha Journal for science 20(1):88-99(2015).
Chemical profiles as chemotaxonomic tools for some species in Fabaceae in Iraq
Huda Jasim Mohammed Al-Tameme
University of Babylon / College of Science for Women/ Department of Biology
Huda_jasim@yahoo.com
PO box 435, Al-Hillah city, Babylon, Iraq. Tel: 07709829765
Flavonoid contents were investigated in alcoholic extract of fresh leaves in some species belong to family of Fabaceae grown in Iraq which collected randomly from various fields in Hillah city. Flavonoids compounds were isolated by using Thin Layer Chromatography (TLC) in n-Butanol: Acetic acid: Water (BAW) and Ethyl acetate: Methanol: Water (EMW) solvents systems. These compounds were classified according to their Retention factor (Rf) value, color under Ultraviolet (UV) and some references.
TLC profiling of leaves extract give an idea about the presence of various phytochemicals. Different Rf value of various phytochemicals provide valuable clue regarding their polarity and selection of solvents for separation of phytochemicals.
Key words: Fabaceae, TLC technique, Flavonoid, BAW, EMW, Mimosoideae, Caesalpinoideae, Papilionoideae
Introduction
Chemotaxonomy is the systematic study of chemical variation between plant taxa. Evidence of chemical variation has essentially been used for classification purposes ever since folk taxonomies based on certain obvious plant characteristics were instinctively employed by mankind centuries ago [1] .