clotting time

Laboratory Diagnosis of Clotting Disorders
Clotting Time Test (C.T) The C.T determines the ability of capillary blood to clot within a time, after a puncture of the capillaries
Equipments:-
1-Un - Heparinized capillary tube (Blue color).
2-Lancet
3-Watch
Specimen:-
The specimen must be used is the capillary blood.
Procedure:-
1-Sterilize the surface of finger.
2-Prick by lancet and record the time.
3- Collect the blood with un-heparinized capillary tube.
4- Move the capillary tube between your fingers in sloping, until the blood moving is stopped.
5-Break of a small piece of capillary tubes every 33 sec. until you see the strand of fibrin.
6- Stop the watch, record the total time. This represents the C.T expressed in min. Normal value: - C.T = 2 – 11 min.
Bleeding Disorders Laboratory Diagnosis of Clotting Disorders
Clotting Time Test (C.T) The C.T determines the ability of capillary blood to clot within a time, after a puncture of the capillaries
Equipments:-
1-Un - Heparinized capillary tube (Blue color).
2-Lancet
3-Watch
Specimen:-
The specimen must be used is the capillary blood.
Procedure:-
1-Sterilize the surface of finger.
2-Prick by lancet and record the time.
3- Collect the blood with un-heparinized capillary tube.
4- Move the capillary tube between your fingers in sloping, until the blood moving is stopped.
5-Break of a small piece of capillary tubes every 33 sec. until you see the strand of fibrin.
6- Stop the watch, record the total time. This represents the C.T expressed in min. Normal value: - C.T = 2 – 11 min.
Bleeding Disorders
The bleeding disorders associated with abnormalities in the quality and quantity of the coagulation.
These abnormalities are due to structure of blood vessels or impaired platelets function, like a reduction in their number in the blood stream, or disorders of plasma proteins involved in coagulation.
Abnormal synthesis of coagulation factors will result in a bleeding disorder known as Hemophilia.
There are many different forms of hemophilia, each of which results from the lack of one or more clotting factors, this leads to in some conditions to spontaneous bleeding into the muscles or joints and may produce a local gangrene.
Some dietary reasons can contribute in the disorder of bleeding such as vit.k deficiency, which is essential for synthesis and activation of some coagulation factors. Also in some conditions that the person requires transfusion of large amount of stored blood tend to infect with defects of bleeding because when blood is stored some clotting factors undergo chemical changes and become inactive, also the platelets tend to disappear from the stored blood after 4 - 5 days, and fall in their numbers cue to the dilution of the patient s blood and from increased bleeding